ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2016, Vol. 47 ›› Issue (4): 789-795.doi: 10.11843/j.issn.0366-6964.2016.04.019

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Expression of Mycobacterium avium subsp. paratuberculosis MAP0862 and Its Primary Application in Indirect ELISA

ZHANG Zhen1,2,FENG Yu1,ZHANG Ge2,ZHU Liang-quan2,JIANG Hui2,DING Jia-bo2* ,ZHAO Peng1,CHANG Wei-shan1*   

  1. (1.College of Animal Science and Veterinary Medicine,Shandong Agricultural University,Tai’an 271018,China;2.China Institute of Veterinary Drug Control,Beijing 100081,China)
  • Received:2015-07-27 Online:2016-04-23 Published:2016-04-23

Abstract:

The objective of the present study was to explore the effect of MAP0862 in sero-diagnosis of Bovine paratuberculosis,and establish a specific ELISA method based on MAP0862.The specific protein obtained by prokaryotic expression was used as coating antigen after purification and quantification,an indirect ELISA was primary established after a series of optimization.The specificity of this method was verified by MAP antiserum,Brucella antiserum,Bovine tuberculosis (TB) antiserum,BCG antiserum,Escherichia coli antiserum and negative serum.The detection results of 300 serums showed that the coincidence rate of MAP0862 indiret ELISA with commercial kit was 94.3%.The result indicated that the indirect ELISA method primary established based on MAP0862 can detect Bovine paratuberculosis effectively.

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